Semi Prep HPLC Column, Bidentate C8, 4um, 100A. 10mm ID x 100mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑SP100 Cogent Bidentate C8™ Semi‑Preparative HPLC Column is a longer, large‑diameter purification column designed for applications requiring enhanced resolution and higher purity fraction collection compared to shorter semi‑prep formats. Built on Cogent TYPE‑C™ silica hydride technology, this column provides excellent selectivity, rapid equilibration, and very robust column lifetime under demanding purification workflows.
The stationary phase consists of an aliphatic C8 hydrocarbon chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This creates a phase that is more hydrophobic than conventional C8 columns, yet less hydrophobic than Bidentate C18™, making it ideal for hydrophobic molecules, metabolites, and amine‑containing analytes when C18 retention is excessive.
With a 10 mm internal diameter and 100 mm length, this column delivers increased plate count and improved separation efficiency compared with 50 mm semi‑prep formats. This allows better resolution of closely eluting compounds while still supporting large injection volumes and high mass loading for purification workflows.
System Note: A specialized semi‑preparative HPLC system (appropriate flow‑rate capability, injector, tubing/valving, and fraction‑collection hardware) is required for columns of this size.
Why Choose the 10 × 100 mm Semi‑Prep Bidentate C8™ Format
This configuration is commonly selected when:
C18 columns are overly retentive for target analytes
Greater resolution is required than 10 × 50 mm formats provide
Large sample volumes must be purified with improved separation
Hydrophobic or basic analytes benefit from moderated retention
The 100 mm length increases interaction time and selectivity, while TYPE‑C™ silica hydride chemistry maintains method robustness and reproducibility.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Non‑traditional reversed‑phase chemistry
Reduced silanol activity and improved peak shape
Faster equilibration than conventional prep C8 columns
Very robust lifetime for repeated purification runs
Bidentate C8™ Bonding
Two‑point attachment via direct Si–C bonds
Balanced hydrophobic retention for semi‑prep applications
Ideal alternative when C18 is overly retentive
Semi‑Preparative Geometry with Enhanced Resolution
10 mm ID × 100 mm length
Increased plate count vs. 50 mm columns
Supports higher purity fraction collection
Broad Method Compatibility
Stable at very low pH (below pH 2)
Compatible with aqueous‑rich mobile phases
Suitable for demanding purification conditions
Typical Applications
Semi‑preparative purification
Fraction collection with improved purity
Hydrophobic molecule isolation
Metabolite purification
Amine‑containing analytes
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable